High-yield continuous lentiviral vector bioprocessing through perfusion intensification and integrated cold chain affinity capture
Perfusion intensification, cold harvest stabilization, and inline affinity capture together can more than double lentiviral vector (LVV) recovery while removing steps from the process. This session presents a continuous LVV workflow that reached around 12 × 10⁶ viable cells/mL before transfection and delivered roughly 16-fold higher cumulative transduction units over 3 days through cold harvest handling.
Keen Chung, Technical Lead for Viral Vector Culture and Analytics at Repligen, will show how coupling affinity capture directly to the perfusion permeate eliminates clarification and bulk harvest storage, cutting hold times, contamination risk, and product loss.
Attend this webinar to:
- Apply a perfusion intensification approach that reached around 12 × 10⁶ viable cells/mL before transfection, roughly 4-fold higher cell density, to raise vector yield and cell-specific productivity
- Quantify how continuous cold harvest at 4°C delivered around 16-fold higher cumulative transduction units over 3 days, informed by an LVV infectivity half-life of around 6 h at 37°C versus around 300h at 4°C
- See how affinity capture coupled directly to the permeate enables continuous, hold-free purification and removes clarification and bulk storage as separate unit operations
Compare overall recovery against a conventional depth filtration and anion exchange chromatography route (around 25%), which the integrated process improved more than 2.5-fold